Protective Effects of Omega-3 Fatty Acids on Energy Drink Induced Ovarian Cytotoxicity in Adult Female Albino Rats:

Energy Drinks Induced Cytotoxicity: A Randomized Controlled Trial

Authors

  • Maria Ilyas Al Aleem Medical College, Lahore, Pakistan
  • Masooma Ahmad Al Aleem Medical College, Lahore, Pakistan
  • Huma Jawad Al Aleem Medical College, Lahore, Pakistan
  • Nazia Siddique Al Aleem Medical College, Lahore, Pakistan
  • Lubna Shahper Ameer-ud-Din Medical College, Lahore, Pakistan
  • Muhammad Hashim Ghouri Institute of Physiotherapy, Gulab Devi Hospital, Lahore, Pakistan

DOI:

https://doi.org/10.37978/tijfs.v5i1.329

Keywords:

Caffeine ; Carbonated Beverages; College Students; Community Health Education; Cytotoxicity; Cytotoxins; Docosahexaenoic Acids; Eicosapentaenoic Acid; Energy drinks; Energy Intake; Energy Metabolism; Global health; Health Education; Infertility; Omega-3; Ovarian Diseases; Oxidative stress; Preventive Medicine; Public Health; Women’s health.

Abstract

Background: Energy drinks (EDs) are commonly used to prevent fatigue, enhance physical, and cognitive performance. Its administration induces toxic effects in body. Omega-3 is an antioxidant and anti-inflammatory agent that helps in proper functioning of immune system. Objectives of this study were to evaluate the morphological effects of fish oil omega 3 fatty acids (Eicosapentaenoic acid / Docosahexaenoic acid) on energy drink induced ovarian cytotoxicity in adult female albino rats.

Methods: The study was conducted at animal house, Anatomy department, Postgraduate Medical Institute, Lahore from January to march 2019. ARRIVE guidelines were followed for conduct of animal study. Ethical approval was obtained from PGMI, Lahore and Advanced Studies and Research Board of University of Health Sciences, Lahore. The study comprised 36 adult female albino rats divided into 3 groups i.e., control, energy drink and omega 3 treated. Rats were sacrificed, ovaries extracted, and sections were stained with H&E and PAS. SPSS version 21.0 were used.

Results: Statistically significant difference was present in gross parameters between the control and experimental groups. Energy drink administration caused a decrease in diameter of mature graafian follicle and diameter of the oocyte. Disruption in basement membrane was more pronounced in Energy drink treated group.

Conclusion: Energy drinks were found to cause cytotoxic effects on ovarian and oocyte morphology, ultimately leading to infertility. Omega 3 reduces the extent of damage caused by the intake of energy drinks.

Downloads

Download data is not yet available.

Author Biographies

Maria Ilyas, Al Aleem Medical College, Lahore, Pakistan

Dr Maria Ilyas, Department of Anatomy, Al Aleem Medical College, Lahore, Pakistan

Masooma Ahmad , Al Aleem Medical College, Lahore, Pakistan

Dr Masooma Ahmad, Assistant Professor, Department of Anatomy, Al-Aleem Medical College Lahore, Pakistan

Huma Jawad, Al Aleem Medical College, Lahore, Pakistan

Dr Huma Jawad, Assistant Professor, Department of Anatomy, Al Aleem Medical College, Lahore, Pakistan

Nazia Siddique, Al Aleem Medical College, Lahore, Pakistan

Prof. Dr. Nazia Siddique, Professor, Department of Anatomy, Al Aleem Medical College, Lahore, Pakistan

Lubna Shahper, Ameer-ud-Din Medical College, Lahore, Pakistan

Dr Lubna Shahper, Department of Anatomy, Ameer-ud-Din Medical College, Lahore, Pakistan

Muhammad Hashim Ghouri, Institute of Physiotherapy, Gulab Devi Hospital, Lahore, Pakistan

Dr Muhammad Hashim Ghouri, PT, President Pakistan Research & Welfare Society; Institute of Physiotherapy, Gulab Devi Hospital, Lahore, Pakistan

Published

2021-01-17

How to Cite

Ilyas, M., Ahmad , M., Jawad, H. ., Siddique, N. ., Shahper, L., & Ghouri, M. H. (2021). Protective Effects of Omega-3 Fatty Acids on Energy Drink Induced Ovarian Cytotoxicity in Adult Female Albino Rats: : Energy Drinks Induced Cytotoxicity: A Randomized Controlled Trial. The International Journal of Frontier Sciences, 5(1). https://doi.org/10.37978/tijfs.v5i1.329