Protective Effects of Omega-3 Fatty Acids on Energy Drink Induced Ovarian Cytotoxicity in Adult Female Albino Rats:
Energy Drinks Induced Cytotoxicity: A Randomized Controlled Trial
Keywords:Caffeine ; Carbonated Beverages; College Students; Community Health Education; Cytotoxicity; Cytotoxins; Docosahexaenoic Acids; Eicosapentaenoic Acid; Energy drinks; Energy Intake; Energy Metabolism; Global health; Health Education; Infertility; Omega-3; Ovarian Diseases; Oxidative stress; Preventive Medicine; Public Health; Women’s health.
Background: Energy drinks (EDs) are commonly used to prevent fatigue, enhance physical, and cognitive performance. Its administration induces toxic effects in body. Omega-3 is an antioxidant and anti-inflammatory agent that helps in proper functioning of immune system. Objectives of this study were to evaluate the morphological effects of fish oil omega 3 fatty acids (Eicosapentaenoic acid / Docosahexaenoic acid) on energy drink induced ovarian cytotoxicity in adult female albino rats.
Methods: The study was conducted at animal house, Anatomy department, Postgraduate Medical Institute, Lahore from January to march 2019. ARRIVE guidelines were followed for conduct of animal study. Ethical approval was obtained from PGMI, Lahore and Advanced Studies and Research Board of University of Health Sciences, Lahore. The study comprised 36 adult female albino rats divided into 3 groups i.e., control, energy drink and omega 3 treated. Rats were sacrificed, ovaries extracted, and sections were stained with H&E and PAS. SPSS version 21.0 were used.
Results: Statistically significant difference was present in gross parameters between the control and experimental groups. Energy drink administration caused a decrease in diameter of mature graafian follicle and diameter of the oocyte. Disruption in basement membrane was more pronounced in Energy drink treated group.
Conclusion: Energy drinks were found to cause cytotoxic effects on ovarian and oocyte morphology, ultimately leading to infertility. Omega 3 reduces the extent of damage caused by the intake of energy drinks.
How to Cite
Copyright (c) 2021 The International Journal of Frontier Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.