Laboratory Detection of Novel Corona Virus 2019 using Polymerase Chain Reaction


  • Shafiq Ahmad Chudhary Institute of Biomedical and Allied Health Sciences, University of Health Sciences, Lahore, Pakistan
  • Shahid Imtiaz Department of Microbiology and Molecular Genetics, University of Punjab, Lahore, Pakistan
  • Nedal Iqbal Department of Oral Biology, FMH College of Medicine and Dentistry, Lahore, Pakistan



2019-nCoV, Laboratory Diagnosis, RT-PCR


Since mid-December 2019, several cases of a pneumonia like disease (with symptoms including fever, difficulty in breathing, cough and invasive lesions on both lungs) of unknown cause have emerged in the central Chinese city of Wuhan. Chinese authorities made a preliminary determination that the causative agent is a novel coronavirus(2019-nCoV). Coronaviruses are enveloped RNA viruses belonging to Coronaviridae family and the order Nidovirales. This subfamily consists of four genera alphacoronavirus, betacoronavirus, gammacoronavirus and deltacoronavirus on the basis of their phylogenetic relationships and genomic structures. These subfamilies are broadly distributed for causing infections in humans and other mammals. The alphacoronaviruses and betacoronaviruses infect only mammals. The gammacoronaviruses and deltacoronaviruses infect birds, but some of them can also infect mammals. The source of betacoronavirus 2019-nCoV is still unknown, although initial cases have been linked with south Huanan seafood market. Viral infections already known to produce similar symptoms are influenza, parainfluenza, Middle East respiratory syndrome (MERS-CoV) and severe acute respiratory syndrome (SARS-CoV). Laboratory investigations reported raised plasma levels of L2, IL7, IL10, GSCF, IP10, MCP1, MIP1A, and TNF? in patients.


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How to Cite

Chudhary, S. A., Imtiaz, S., & Iqbal, N. (2020). Laboratory Detection of Novel Corona Virus 2019 using Polymerase Chain Reaction. The International Journal of Frontier Sciences, 4(2), 101.



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